Their union says more than 40,000 employees of Network Rail and 15 train operators will go on strike once more on October 8.
As part of a protracted dispute over salary, employment, and working conditions, the RMT declared that it will “effectively shut down the railway network.”
It will come just a week after an even bigger strike by members of RMT and the train drivers union Aslef.
Network Rail warned passengers to expect very significant disruption and only travel if absolutely necessary.
It added that full timetables for upcoming strike days would be published soon.
The latest action comes after a series of large-scale walkouts as unions call for pay increases in line with the rising cost of living.
In June, the biggest rail strikes for 30 years threatened to grind the country to a halt. But while some people faced substantial disruption it was business as usual for others.
RMT general secretary Mick Lynch said the union had met with new Transport Secretary Anne-Marie Trevelyan to discuss the disputes.
“However, as no new offer has been tabled, our members have no choice but to continue this strike action,” he said.
“We will continue to negotiate in good faith, but the employers and government need to understand our industrial campaign will continue for as long as it takes.”
The RMT, which represents rail workers including guards and signalling staff, had already announced its 40,000 members would strike on 1 October. This is the day before the London Marathon and the Conservative Party conference in Birmingham.
Train drivers at 12 operators who are members of Aslef will also walk out on 1 October, meaning that only 10% of UK services are likely to run that day.
Aslef members will also hold a second day of strike action on 5 October.